PDF《火焰原子吸收法测定乳粉中的锰》

第10 卷第11 期食品安全质量检测学报Vol.

10 No.

11 2019 年6月Journal of Food Safety and Quality Jun. ,

2019 *通讯作者: 那斯琴高娃, 工程师, 主要研究方向为食品仪器分析检测.E-mail: gaowa@mengniu.cn *Corresponding author: NASIQIN Gao-Wa, Engineer, Inner Mongolia Mengniu Dairy Industrial Co., Ltd., Hohhot 011500, China. E-mail: gaowa@mengniu.cn 火焰原子吸收法测定乳粉中的锰 那斯琴高娃* , 常建军, 宋晓东 (内蒙古蒙牛乳业集团股份有限公司, 呼和浩特 011500) 摘要: 目的 优化火焰原子吸收法测定乳粉中锰的分析方法.方法 对火焰原子吸收分光光度计仪器的燃 烧器高度、助燃比等仪器条件进行优化, 确定最佳实验条件.结果 最佳仪器条件: 灯电流 7.5 mA;

狭缝宽 度: 0.2 nm;

燃烧器高度

10 mm;

燃气流量: 2.4 L/min, 助燃气流量: 15.0 L/min.在最佳仪器条件下乳粉中的 锰0~3 mg/L 范围内线性关系良好, 相关系数在 0.9995 以上, 检出限为 0.0099 mg/kg、 回收率为 93.6%~99.5%, 精密度为 0.66%~3.49%.结论 本方法具有灵敏度高、准确度高、精密度好等优点, 适用于乳粉中锰的含 量测定. 关键词: 火焰原子吸收法;

锰;

乳粉 Determination of manganese in milk powder by flame atomic absorption spectrometry NASIQIN Gao-Wa* , CHANG Jian-Jun, SONG Xiao-Dong (Inner Mongolia Mengniu Dairy Industrial Co., Ltd., Hohhot 011500, China) ABSTRACT: Objective To optimize the method for the determination of manganese in milk powder by flame atomic absorption spectrometry. Methods The burner height, combustion ratio and other instrumental conditions of flame atomic absorption spectrophotometer were optimized to determine the best experimental conditions. Results Best instrument conditions were as follows: lamp current was 7.5 mA, slit width was 0.2 nm, burner height was

10 mm, gas flow rate was 2.4 L/min, auxiliary gas flow rate was 15.0 L/min. Under the optimal instrument condition, manganese in milk had good linear relationships in the range of 0-3 mg/L, and the correlation coefficients were larger than or equal to 0.9995. The limit of detection was 0.0099 mg/kg. The recoveries were 93.6%-99.5%, with the relative standard deviations of 0.66%-3.49%. Conclusions This method has the advantages of high sensitivity, high accuracy and good precision, which is suitable for the determination of manganese in milk powder KEY WORDS: flame atomic absorption spectrometry;

manganese;

milk powder

1 引言锰是正常机体必需的微量元素之一, 对人体健康有 着重要作用.它可促进骨骼的生长发育, 保护细胞中细粒 体的完整, 保持正常的脑功能, 维持正常的糖代谢和脂肪 代谢, 改善肌体的造血功能[1] . 目前锰含量的检测方法主要有火焰原子吸收法、 石墨 炉原子吸收法和电感耦合等离子体发射光谱法[2] .石墨炉 原子吸收法和电感耦合等离子体发射光谱法检测时间长、 成本高、重现性差.火焰原子吸收法具有重现性好、检测 成本低、检测时间短等优点[3] .本研究依据食品中锰的测 定国标方法, 采用火焰原子吸收法测定奶粉中锰含量, 优第11 期 那斯琴高娃, 等: 火焰原子吸收法测定乳粉中的锰

3601 化仪器条件, 确定适合分析奶粉中锰的最优条件, 以期为 乳粉企业食品安全风险监控方法提供参考依据.

2 材料与方法 2.1 仪器Z-2000 原子吸收分光光度计、锰空心阴极灯(日本日 立公司);

L24/12/P320 马弗炉(德国 Nabertherm 公司). 2.2 试剂硝酸(优级纯, 北京化工厂);