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488 goat anti- rabbit as a secondary antibody (green). b: DAPI stained U20S nuclei (blue). c: Actin stained with Alexa Fluor?

594 phalloidin (red). d: Composite image of cells showing cytoplasmic localization of TIMP4. Explanation of Symbols Symbol Description Symbol Description Symbol Description Manufacturer Catalog number Batch code Use by Temperature limitation Consult instructions for use Caution, consult accompanying documents DISCLAIMER: LIFE TECHNOLOGIES CORPORATION AND/OR ITS AFFILIATE(S) DISCLAIM ALL WARRANTIES WITH RESPECT TO THIS DOCUMENT, EXPRESSED OR IMPLIED, INCLUDING BUT NOT LIMITED TO THOSE OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, OR NON-INFRINGEMENT. TO THE EXTENT ALLOWED BY LAW, IN NO EVENT SHALL LIFE TECHNOLOGIES AND/OR ITS AFFILIATE(S) BE LIABLE, WHETHER IN CONTRACT, TORT, WARRANTY, OR UNDER ANY STATUTE OR ON ANY OTHER BASIS FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING BUT NOT LIMITED TO THE USE THEREOF. NOTICE TO PURCHASER: Limited Use Label License No. 327: Recombinant Antibody Technology: The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research and manufacturing conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product, (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. The buyer may transfer information or materials made through the use of this product to a scientific collaborator, provided that such transfer is not for any Commercial Purpose, and that such collaborator agrees in writing (a) not to transfer such materials to any third party, and (b) to use such transferred materials and/or information solely for research and not for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components for contract manufacturing services;

(2) use of the product or its components to provide a service, information, or data;

(3) use of the product itself or its components as a therapeutic, diagnostic or prophylactic;

or (4) resale of the product or its components, whether or not such product or its components are resold for use in research or manufacturing. If the purchaser is not willing to accept the limitations of this limited use statement, Life Technologies is willing to accept return of the product for a full refund. For information on obtaining additional rights, please contact outlicensing@lifetech.com. ?

2012 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation and/or its affiliates or their respective owners. For support visit lifetechnologies.com/support or email techsupport@lifetech.com lifetechnologies.com

3 December 2012

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