PDF《农药残留检测中不同蔬菜的基质效应》

? 研究论文 ? DOI: 10.

16801/j.issn.1008-7303.2019.0040 农药残留检测中不同蔬菜的基质效应 张圆圆, 刘磊, 李娜, 李辉, 卢娜, 郭永泽, 张玉婷* (天津市农业质量标准与检测技术研究所,农业农村部农产品质量安全风险评估实验室 (天津),天津 300381) 摘要:基于 QuEChERS 前处理方法,在气相色谱-串联质谱 (GC-MS/MS) 和超高效液相色谱- 串联质谱 (UPLC-MS/MS) 检测条件下,考察了

200 种农药在

17 种蔬菜基质中的基质效应,通 过基质效应分析找到了合适的代表性基质用作配制检测过程中的基质匹配标准溶液.结果表 明:由于基质干扰成分及农药性质的差异,导致不同蔬菜及农药品种之间的基质效应差异.在GC-MS/MS 检测中,供试的

150 种农药中绝大部分表现为基质增强效应,其中葱、姜和大蒜表 现为很强的基质效应,萝卜的基质效应较弱;

菠菜、芹菜、豇豆和生菜

4 种蔬菜对其他蔬菜品 种能够起到很好的基质校正效果,可作为代表性基质在日常检测中用于其他蔬菜基质的定量分 析.在UPLC-MS/MS 检测中,供试的

105 种农药中大部分表现为基质抑制效应,其中姜、大蒜、葱、芹菜、韭菜和菠菜的基质效应较强,西葫芦的基质效应弱;

黄瓜、普通白菜、番茄和 生菜

4 种蔬菜能较好地校正其他蔬菜的基质效应,但与 GC-MS/MS 相比校正能力稍弱.本研究 结果认为代表性蔬菜基质可用于降低基质效应对检测结果的干扰. 关键词: 蔬菜;

农药残留;

基质效应;

超高效液相色谱-串联质谱;

气相色谱-串联质谱 中图分类号: S481.8;

TQ450.263 文献标志码:A 文章编号:1008-7303(2019)03-0327-11 Matrix effects in pesticide residue analysis in various vegetable samples ZHANG Yuanyuan, LIU Lei, LI Na, LI Hui, LU Na, GUO Yongze, ZHANG Yuting* (Tianjin Institute of Agricultural Quality Standard and Testing Technology, Laboratory of Quality &

Safety Risk Assessment for Aro-products(Tianjin), Ministry of Agriculture and Rural Affairs, Tianjin 300381, China) Abstract: In this paper, the matrix effects on the residue analysis of

200 pesticides under gas chromatography-tandem mass spectrometry (GC-MS/MS) and ultra high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) conditions were investigated.

17 different vegetable matrices based on the QuEChERS preparation were studied. The study is aimed to find the typical vegetables as blank matrices to compensate the matrix effects in pesticide residue analysis. The results showed that differences in matrix interferences and pesticides characteristics lead to varied matrix effects in and between GC-MS/MS and UPLC-MS/MS analysis. In GC-MS/MS analysis, the majority of the

150 pesticides exhibited matrix enhancement effects. The matrix effects of green onion, ginger and garlic were strong. Radish exhibited weak matrix effect. The matrix effects of the majority of vegetables could be well compensated by spinach, celery, cowpea and lettuce, and these four typical 农药学学报 2019, 21(3): 327-337 Chinese Journal of Pesticide Science http://nyxxb.alljournal.cn 收稿日期:2018-12-15;

录用日期:2019-03-13. 基金项目:国家重点研发计划 (2016YFD080100703);

天津市蔬菜产业体系创新团队 (ITTVRS2017021);

天津市重点研发计划项目 (17YFXTZC00040). 作者简介:张圆圆,女,硕士,研究实习员,研究方向为农产品质量安全,E-mail:zyyzyyok@163.com;

* 张玉婷,通信作者 (Author for correspondence),女,学士,研究员,主要从事农产品质量安全研究,E-mail:yutingtj@163.com vegetables can be used in daily analysis as blank matrices. In the UPLC-MS/MS analysis, most of the