PDF《口服液类保健食品中 18 种重金属元素检测》

现代食品科技 Modern Food Science and Technology 2019, Vol.

35, No.2

285 口服液类保健食品中

18 种重金属元素检测 程敏 1,2 ,展敏 3,4 ,谭丽容 1,2 ,严诗楷 3,5 ,李莎莎

4 ,肖雪

3 (1.弘正道(中国)中药研究有限公司,广东广州 510665)(2.无限极(中国)有限公司,广东江门 529156) (3.广东药科大学中医药研究院,广东广州 510006)(4.广东省中医院,广东广州 510006) (5.上海交通大学药学院,上海 200240) 摘要:本文旨在建立一种基于微波消解-电感耦合等离子质谱(Inductively coupled plasma mass spectrometry,ICP-MS)测定源于 中草药的口服液类保健食品中

18 种重金属元素(Pb、Cd、As、Hg、Cu、Pt、Pd、Ir、Rh、Ru、Os、Mo、Ni、Cr、V、Mn、Fe、Zn) 的方法.采用微波消解法处理口服液样品,以Ge、In、Bi 元素作为校正基体效应与信号漂移的内标元素,使用 ICP-MS 对十批样品 中18 种重金属进行含量测定. 结果显示

18 种重金属标准曲线线性关系良好, R2 ≥0.999, 各元素的检出限在 0.0001~2.743 ng/mL 之间, 精密度 RSD 在0.49%~4.29%之间,重复性 RSD 值在 1.2%~15%之间,加样回收率在 82.74%~122.7%之间;

检测两种口服液中

18 种 重金属元素均未超标.表明该方法操作简便、分析速度快、灵敏度高、准确性好,适用于口服液类保健食品中重金属检测,可为保健 食品安全性评价提供依据. 关键词:电感耦合等离子质谱;

重金属检测;

中草药;

保健食品 文章篇号: 1673-9078(2019)02-285-290 DOI: 10.13982/j.mfst.1673-9078.2019.2.040 Study on

18 Heavy Metal Elements in Oral Liquid Health Foods CHENG Min1,2 , ZHAN Min3,4 , TAN Li-rong1,2 , YAN Shi-kai3,5 , LI Sha-sha4 , XIAO Xue3 (1.Hong Zheng Dao (China) Traditional Chinese Medicine Research Co. Ltd., Guangzhou 510665, China) (2.Infinitus (China) Co. Ltd, Jiangmen 529156, China) (3.Institute of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China) (4.Guangdong Hospital of Chinese Medicine, Guangzhou 510006, China)(5.School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China) Abstract: A kind of heavy metal elements (Pb, Cd, As, Hg, Cu, Pt, Pd, Ir, Rh, Ru, Os, Mo, Ni, Cr, V, Mn, Fe, Zn) in oral liquid health foods derived from Chinese herbal medicine based on inductively coupled plasma mass spectrometry (ICP-MS) was established in this work. The oral liquid samples were pre-treated by microwave digestion. The Ge, In and Bi elements were used as the internal standard elements for correcting the matrix effect and signal drift. The contents of

18 heavy metals in ten batches of samples were determined by ICP-MS. Results showed that good linear relations of

18 heavy metal standard curves were found, R2 ≥0.999, the detection limit of each element was between 0.0001~2.743 ng/mL, the precision RSD was between 0.49%~4.29%, and the repetitive RSD value was between 1.2% and 15%, the recovery rate of the sample was between 82.74% and 122.7%. The

18 heavy metal elements in the two oral liquids were not exceeded. Results showed that this method is easy to operate, fast in analysis, high in sensitivity and good in accuracy. It is suitable for the detection of heavy metals in oral liquid health foods, and can provide a basis for health food safety evaluation. Key words: inductively coupled plasma mass spectrometry;

heavy metal detection;

Chinese herbal medicine;

healthy food 保健食品,声称并具有特定保健功能或者以补充 维生素、矿物质为目的的食品.即适用于特定人群食 用,具有调节机体功能,不以治疗疾病为目的,并且 对人体不产生任何急性、 亚急性或慢性危害的食品[1] . 在国内,源于中草药原料的保健食品一直是研发和市 场热点.然而受土壤、水源、大气污染等影响,中草 收稿日期:2018-09-27 作者简介:程敏(1979-) ,男,工程师,本科,研究方向:中药质量评价 通讯作者:李莎莎(1983-) ,女,博士,助理研究员,研究方向:中医药系 统生物学 药可能存在重金属和有害元素超标的风险,这就有可 能对这类产品产生一定的影响.彭启志[2] 对五个不同 中药材种植基地土壤、中药材样中 Pb、Cd、As 和Hg 含量进行调查与评价,经过调查分析,作者发现不同 中药材种植基地土壤重金属污染日趋严重.黄柏、杜 仲和板蓝根重金属含量都有增加,其中 Pb、Cd 和As 含量增加最为明显.雷汀菲等[3] 测定了六种常见中草 药中 Pb 和Cd 的含量, 发现六种中草药中都含有一定 量的 Pb 和Cd,板蓝根重金属含量最高,其次是地黄 和黄芪.经统计,现可有