PDF《超高效液相色谱法检测啶虫脒在工厂化 双孢蘑菇栽培中的残留》

? 研究论文 ? DOI: 10.

16801/j.issn.1008-7303.2016.0051 超高效液相色谱法检测啶虫脒在工厂化 双孢蘑菇栽培中的残留 张其才1,2 , 董茂锋1 , 宋卫国*,1 , 付白莲1,2 , 王伟民1 , 赵志辉1 , 王坦1 , 陈珊珊1 , 曲明清1 , (1. 上海市农业科学院 农产品质量标准与检测技术研究所/上海市农产品质量安全评价技术服务平台, 上海 201403;

2. 上海海洋大学 食品学院,上海 201306) 摘要:采用超高效液相色谱法 (UPLC-PDA) 测定了双孢蘑菇及其覆土和培养料中啶虫脒的残 留量,研究了啶虫脒在工厂化双孢蘑菇栽培中的残留规律.样品以乙腈提取,分散固相萃取净 化,超高效液相色谱测定.结果表明:在0.02~3 mg/kg

3 个添加水平下,啶虫脒在双孢蘑菇、 覆土和培养料

3 种基质中的平均添加回收率为 81%~97%,相对标准偏差 (RSD) 为5.7%~9.6%.在不同施药水平下,啶虫脒在覆土和培养料中的消解动态均符合一级动力学指数 方程,平均半衰期分别为

34 和23 d.覆土单独施药后啶虫脒会向培养料迁移,培养料内的啶 虫脒残留量总体先升高后降低.随覆土或培养料中啶虫脒施药剂量的增加,双孢蘑菇中啶虫脒 的残留量随之增加,当啶虫脒在覆土或培养料中的施药量有效成分在 10~250 mg/kg 之间时,双 孢蘑菇子实体中啶虫脒的残留量在 0.03~2.6 mg/kg 之间,且第一潮、第二潮和第三潮菇间残留 量存在显著差异.同一处理剂量下,覆土单独施药后蘑菇中啶虫脒的残留量显著高于培养料单 独施药后蘑菇中的残留量. 关键词: 超高效液相色谱;

啶虫脒;

双孢蘑菇;

消解;

残留 中图分类号:S482.3 文献标志码:A 文章编号:1008-7303(2016)03-0373-07 Determination of acetamiprid residue in industrial cultivation of Agaricus bisporus by ultra performance liquid chromatography ZHANG Qicai1,2 , DONG Maofeng1 , SONG Weiguo*,1 , FU Bailian1,2 , WANG Weimin1 , ZHAO Zhihui1 , WANG Tan1 , CHEN Shanshan1 , QU Mingqing1 (1. Institute of Agro-food Standard and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai Engineering Research Center of Agro-food Quality and Safety, Shanghai 201403, China;

2. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China) Abstract: The transformation of acetamiprid during Agaricus bisporus industrial production was investigated by testing its residues in cover-soil, compost and fruits using ultra performance liquid chromatography (UPLC-PDA). The samples were extracted with acetonitrile and purified by dispersive 农药学学报 2016, 18(3): 373-379 Chinese Journal of Pesticide Science http://www.nyxxb.com.cn 收稿日期:2015-11-25;

录用日期:2016-04-07. 基金项目:国家自然科学基金项目 (31301699);

公益性行业 (农业) 科研专项(201303088). 作者简介:张其才,男,硕士,研究方向为农药残留安全评价;

* 宋卫国,通信作者 (Author for correspondence),男,博士,副研究员, 主要从事农药残留风险评估技术研究,E-mail:sunvegous@hotmail.com solid phase. Acetamiprid residue was then determined by UPLC-PDA. At 0.02-3 mg/kg spiked levels, the average recoveries for cover-soil, compost and fruits were from 81% to 97% (RSDs ≤9.6%). In different application levels, the dissipation of acetamprid in soil and compost was found to conform to the first order kinetics equation and the average half-life was

34 d in cover-soil and

23 d in compost. The acetamprid applied in cover-soil moved into compost. And residues in the compost increased in the first 21-28 d and then started to decrease. At the application rate of 10-250 mg a.i./kg in soil or compost, acetamprid residues in fresh mushroom fruits were 0.03-2.6 mg/kg. And there were significant residue differences among the first, second and third harvesting time stages. At the same pesticide concentration, the acetamiprid residue level in fruit bodies is significantly higher when the pesticide was applied in the cover-soil compared to that when applied in the compost. Keywords: ultra performance liquid chromatography;