DOC《Table S6 Primers used to amplify exons of GAK gene.》

Table S6 Primers used to amplify exons of GAK gene.

Gene Forward primer Reverse primer Size(bp) The primer for amplifing exon 1-28, Exon5?and Exon3?UTR in Ion Torrent semiconductor sequencing Exon1 CTTGACAGCCGGAAGTTCAT TGCTCCGACTTGACCCTACT

725 Exon2 CTGGGGCCATTATGTTTTGTAG TAGCATTTCTGTAAATAGGGGTCAC

570 Exon3 TGCTTTTGACTTTTCTTGTGTCC AACAACTTCATTCCCAACACTTC

527 Exon4 GTGTGCCTGCCTTTGTCC AGGAGACGATGCAGGTGG

570 Exon5 GAGGGCAGAGCACTGATTG ACCCCTGCATCCTAACTGAG

1346 Exon6 CTCTGTGAAATCCCTCAGCC GCTCCACTTCCTGTTACCTTC

814 Exon7 CCGTAGGAGATTGAGGGAGG GCTGCGGTGAGCCTGAG

593 Exon8-9 GTGTCACCCCTGTTGTTCC TGTGATAGGAGACTTGGGGTTC

1102 Exon10 GAGCACCTGCAACAGCC CTCCTGAACTGCACCAAGAC

548 Exon11 ACAGGGTAGACCTGCCAGTG ACATTGCAGCAAGGGAGG

639 Exon12-13 AGCTGCAGGCTGTCATTTC TGGTGGGAAGCAGTGAGG

1231 Exon14 CCTTCCAGACACGACACTTG CATGCAGGTCAAGGCCAG

583 Exon15 GGCCCTAGCCCTGTTCTTC TAGGATCCATGCACAAGCTG

603 Exon16-17 GAGTAAAAGTGGGAGTATCACGAG CCAAGTGGACCCTCAAACTC

1233 Exon18 CCCGTGAAGCAGCTTGTG ACAGACACACACACCAACGG

522 Exon19 GAAATGATGCTCTTCTGGGG CCATGGGCCTCAGAAATTG

664 Exon20 CAGTGGTGGGTTCCTTGTG AGACTGGGATGAAGAGCGAG

665 Exon21 GGTGTTCCTGCAGCACTTG CCTCAGGATGACTTTCCCAG

987 Exon22 CGGGAGCTACTGGCTTCTC CTGTTCCAGGTTTGGGGATG

628 Exon23 GGAATGCGCAGATGCTTG GGGTGCTGCTGATGCTC

642 Exon24 AGCTGTGAAGCTGTTTTCCC CAGTGCCAGTGTCAGCAAC

559 Exon25 ATCCAGAGTGTTCCCGTCG AAGACAGGGTTGCAAAGGC

695 Exon26 TGGGCAACAGTGATACCTCC CCTGGGGCCTGACTCTG

609 Exon27-28 AACATCTGGCTGAGACGAGG ATCAGCTGTTCCTTCGGGAG

866 Exon5?UTR GGATCCACACTGGAGACACA GGGGTGTTTGCAGAGACTGT

1365 Exon3?UTR CCCACGAAGTCACTCTGGTC CCTTGCTCCTTAGCCAACTG

633 The primer for amplifing the

30 candidate SNPs in exon 27-28 of GAK gene in a DNA sequencing Exon 27-28 TCACGGTGGGGACCCAGGT GACCAGGCACTTCCTCAGCGT 568