PDF《Hyperactivated stallion》

1 Hyperactivated stallion spermatozoa fail to exhibit a rheotaxis-like behaviour, unlike other species Jon?Romero-Aguirregomezcorta1 , Emer?Sugrue1 , Lucía?Martínez-Fresneda1 , David?Newport? ?2 & Sean?Fair1 The journey of spermatozoa through the female genital tract is facilitated by rheotaxis, or the cell's preference to swim against a flow, as well as thigmotaxis, the wall tracking behaviour, which guides them to the site of fertilisation.

The aim of this study was to characterise the rheotactic and thigmotactic response of stallion sperm within a microfluidic channel. Stallion sperm rheotaxis was assessed within the microfluidic channel with regard to: (i)A range of flow velocities, (ii)Varying media viscosity and (iii) Sperm hyperactivation. Sperm distribution across the microfluidic channel was also studied and compared to human and ram sperm. Stallion sperm progressed furthest at a velocity range of 10C30??m/s, with an optimum velocity of 20??m/s.A flow viscosity of 2.5cP or greater reduced sperm rheotaxis (P?