PDF《BD Pharmingen™》

BD Pharmingen? Technical Data Sheet PE Mouse Anti-Human CD14 Product Information Material Number:

561707 Alternate Name: LPS receptor;

LPS-R;

Myeloid cell-specific leucine-rich glycoprotein Size:

25 tests Vol.

per Test:

20 ?l Clone: M5E2 Isotype: Mouse IgG2a, κ Reactivity: QC Testing: Human Tested in Development: Baboon, Rhesus, Cynomolgus, Dog Workshop: III

329 Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide. Description The M5E2 monoclonal antibody specifically binds to CD14, a 53-55 kDa glycosylphosphatidylinositol (GPI)-anchored single chain glycoprotein expressed at high levels on monocytes. Additionally, the anti-CD14 antibody reacts with interfollicular macrophages, reticular dendritic cells, and some Langerhans cells. CD14 has been identified as a high affinity cell-surface receptor for complexes of lipopolysaccharide (LPS) and serum LPS-binding protein, LPB. Profile of peripheral blood monocytes analyzed on a BD FACScan? (BDIS, San Jose, CA) Preparation and Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Application Notes Application Flow cytometry Routinely Tested Suggested Companion Products Catalog Number Name Clone Size

555574 PE Mouse IgG2a, κ Isotype Control

100 tests G155-178

554656 Stain Buffer (FBS)

500 ml (none) Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use

1 * 10^6 cells in a 100-?l experimental sample (a test). 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results. 2. Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols. 3. For fluorochrome spectra and suitable instrument settings, please refer to our Fluorochrome Web Page at www.bdbiosciences.com/colors. 4.

561707 Rev.

1 Page

1 of

2 Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. 5. Source of all serum proteins is from USDA inspected abattoirs located in the United States. 6. An isotype control should be used at the same concentration as the antibody of interest. 7. References Knapp W, Dorken B, Rieber EP, et al, ed. Leucocyte Typing IV. New York: Oxford University Press;

1989:1-1208. (Biology) McMichael AJ, Beverly PCL, Gilks W, et al, ed. Leukocyte Typing III: White Cell Differentiation Antigens. New York: Oxford University Press;

1987. (Clone-specific) Schlossman SF, Boumsell L, Gilks W, et al, ed. Leukocyte Typing V: White Cell Differentiation Antigens. New York: Oxford University Press;

1995. (Biology) Wright SD, Ramos RA, Tobias PS, Ulevitch RJ, Mathison JC. CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein. Science. 1990;

249(4975):1431-1433. (Biology)

561707 Rev.

1 Page

2 of 2