PDF《华东理工大学工程硕士学位论文》

The optimum pretreatment condition was that presoaking solid/liquid ratio was 1:1, sulfuric and usage was 5%(w/w), reaction temperature was

190 o C and reacted for 3min;

The result of enzymatic hydrolysis showed that

50 o C ,pH range at 4.2-5.8 were the optimum conditions for hydrolysis. Increasing agitation rate can improve enzymatic reaction rate, but had no effect for increasing the ultimate sugar yield. According to the analysis of concentrated cost, enzyme cost and material cost, the optimum condition of solids loading and enzyme loading was obtained, at which the total cost above was minimum;

The result of hydrogenolysis showed that corn stover sugar after gradually treating by decoloring and ion exchange, propylene glycol yield increased to 48%, and the final result of hydrogenlysis of stover sugar had no significant difference with the pure glucose. Currently, the technology of the polyols production from corn stover has been applied to industrial production preliminarily. In the study of the utilization of the cassava cellulose, the cellulase cost of different ethanol fermentation process options form cassava cellulose was evaluated. The processes include the direct saccharification and fermentation of original cassava cellulose residues, the direct saccharification and fermentation of pretreated cassava cellulose residues, and the 第IV 页 华东理工大学硕士学位论文 simultaneous co-saccharification and fermentation of cassava starch and cassava cellulose. The results show that the utilization of the cassava cellulose in the first two processes were low with the enzyme cost of 13,602 and 11,659 RMB Yuan per tone of ethanol, respectively. Comparing to the first two processes, the third one demonstrated the lowest enzyme cost at 3,589 RMB Yuan per ton of ethanol, which was less than the ethanol price. The result indicated that the third one was the optimized method and provided a pratical way of cassava cellulose utilization in cassava ethanol industry. Keywords: Lignocellulosic biomass;

Bioconversion;

Hydrogenlysis;

Polyols;

Simultaneous saccharification and ethanol fermentation 华东理工大学硕士学位论文 第V页目录 第1章文献综述.1 1.1 生物炼制综述.1 1.2 糖平台技术.3 1.2.1 木质纤维素预处理.3 1.2.2 酶水解.6 1.2.3 影响糖化反应的因素.7 1.3 单糖炼制多元醇.9 1.3.1 多元醇简介.9 1.3.2 氢解糖类物质制备多元醇研究现状.10 1.4 木薯纤维素发酵生产乙醇的研究现状.12 1.5 本文研究主要内容及意义.13 第2章玉米秸秆单糖制备及氢解生产多元醇.15 2.1 引言.15 2.2 材料与方法.15 2.2.1 实验材料.15 2.2.2 实验方法.17 2.2.3 分析方法.19 2.3 结果与讨论.22 2.3.1 秸秆灰分及秸秆清洗对预处理的影响.22 2.3.2 不同产地玉米秸秆稀酸预处理.28 2.3.4 稀酸预处理玉米秸秆糖化条件的探索.31 2.3.5 用于多元醇炼制的糖浓缩液其浓缩成本、酶成本及原料成本分析.38 2.3.6 秸秆糖液加氢裂解生产多元醇.42 2.4 本章小结.44 第VI 页 华东理工大学硕士学位论文 第3章木薯纤维素乙醇发酵的纤维素酶成本评价.45 3.1 材料与方法.45 3.1.1 原料与试剂.45 3.1.2 实验方法.45 3.1.3 分析方法.46 3.2 结果与讨论.46 3.2.1 木薯酒渣的直接同步糖化与发酵生产乙醇.46 3.2.2 木薯酒渣稀酸预处理后的同步糖化与乙醇发酵.47 3.2.3 木薯块茎的同步淀粉与纤维素糖化生产乙醇.50 3.2.4 木薯酒渣生产乙醇方法的工艺对比.51 3.3 本章小结.52 第4章结论与展望.53 4.1 结论.53 4.2 展望.54 参考文献.55 致谢.63 华东理工大学硕士学位论文 第1页第1章文献综述 1.1 生物炼制综述 人类社会的发展离不开稳定的、可持续的能源及资源的供给,近几十年来,石油、 天然气等化石能源的过度开采及消耗, 使得人类面临着能源危机、 环境污染等重大问题. 对此寻找新的能源替代是当今的研究热点,随着全人类对珍惜能源、保护环境等问题达 成的强烈共识,未来社会的能源需求绝大部分必将依赖于风能、水能、太阳能、核能, 当然也包括生物质能等可再生能源. 生物质能来源于植物的光合作用, 据估计, 纤维素、 半纤维素、 木质素等作为植物生物质的主要成份大约以每年 1500-2000 亿吨的速度再生, 换算成能量相当于石油产量的