PDF《黄瓜萌芽期和苗期耐冷性评价》

黄瓜萌芽期和苗期耐冷性评价 * 苗永美 1,

2 宁宇1曹玉杰

1 沈佳1庞欣1崔利1程春燕

1 陈劲枫 1** (

1 作物遗传与种质创新国家重点实验室 /南京农业大学园艺学院,南京 210095;

2 安徽科技学院生命科学学院,安徽凤阳 233100) 摘要选用

13 份黄瓜材料, 研究萌芽期和苗期共

12 个指标与黄瓜耐冷性的关系, 对材料 进行耐冷性评价.

结果表明:

17 ℃下的相对发芽率、 相对发芽势、 相对胚根长和相对活力指数 在13 份黄瓜材料中差异显著( P <

0. 05) , 并与黄瓜耐冷性的相关关系显著;

苗期

4 ℃处理

2 d 时各材料之间生理差异明显, 冷害指数与恢复后成活率的相关性显著, 而与总蛋白、 电导率、 超氧化物歧化酶( SOD) 、 过氧化物酶( POD) 、 过氧化氢酶( CAT) 和抗坏血酸过氧化物酶 ( APX) 活性的相关性不显著. 通过聚类分析将黄瓜材料耐冷性分为

3 个等级, 通过逐步回归 分析建立了两个生育期黄瓜耐冷性方程, 可根据置信区间估计值对黄瓜的耐冷性进行较好的 评价. 关键词 黄瓜 耐冷性 主成分分析 逐步回归 文章编号

1001 - 9332( 2013)

07 -

1914 -

09 中图分类号 S642.

2 文献标识码 A Evaluation of cucumber'

s chilling tolerance at germination and seedling stages. MIAO Yong- mei1,

2 ,NING Yu1 ,CAO Yu- jie1 ,SHEN Jia1 ,PANG Xin1 ,CUI Li1 ,CHENG Chun- yan1 ,CHEN Jin- feng1 (

1 State Key Laboratory of Crop Genetics and Germplasm Enhancement/College of Horticul- ture,Nanjing Agricultural University,Nanjing 210095,China;

2 College of Life Science,Anhui Sci- ence and Technology University,Fengyang 233100,Anhui,China) . - Chin. J. Appl. Ecol. , 2013,

24 ( 7) :

1914 - 1922. Abstract: A total of

13 cucumber materials were used to study the relationships of cucumber'

s chilling tolerance with the

12 growth indices at germination and seedling stages. There existed sig- nificant differences in the relative germination rate,germination index,hypocotyl length,and vigor index at

17 ℃ among the

13 materials ( P <

0. 05) ,which also significantly correlated to the chill- ing tolerance. At seedling stage,the physiology among the materials after treated at

4 ℃ for

2 days had obvious difference. Chilling injury index had significant correlation with the survival rate after recovery,but less correlation with the soluble protein ( SP) content,electric conductivity ( EC) , and superoxide dismutase ( SOD) ,peroxidase ( POD) ,catalase ( CAT) ,and ascorbate peroxidase ( APX) activities. The chilling tolerance of the materials was classified into three levels by cluste- ring analysis,and the cucumber'

s chilling tolerance equations at the two stages were established through stepwise regression analysis. Based on confidence interval value,the chilling tolerance of cucumber could be well assessed. Key words: cucumber;

chilling tolerance;

principal component analysis;

stepwise regression. * 国家重点基础研究发展计划项目(2009CB119001- 01, 2012CB113900) 、 国家高技术研究发展计划专项( 2012AA100202) 、 国 家自然科学基金项目( 31071801) 和江苏省农业科技自主创新基金项 目( CX( 11) 1002) 资助. **通讯作者. E- mail: jfchen@ njau. edu. cn 2013- 02-

07 收稿, 2013- 05-

21 接受. 黄瓜( Cucumis sativus) 是世界性蔬菜, 也是我国 设施蔬菜栽培的主要作物之一. 黄瓜是喜温作物, 正 常情况下, 黄瓜生育界限温度为