PDF《RayBio? Human Acute Kidney Injury》

Patent Pending Technology User Manual (Revised June 2, 2014) RayBio? Human Acute Kidney Injury Antibody Array G-Series Cat# AAH-AKI-G1-4 RayBio? Human Acute Kidney Injury Antibody Array G-Series Cat# AAH-AKI-G1-8 RayBio? Human Cytokine Antibody Array G-Series Testing Service Cat# AAH-SERV-G Please read manual carefully before starting experiment We provide you with excellent Protein Array systems and services Tel: (Toll Free) 1-888-494-8555 or +1-770-729-2992;

Fax: +1-770-206-2393;

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raybiotech.com Email: info@raybiotech.com RayBio? Human Acute Kidney Injury Antibody Array

1 (G-Series) RayBiotech, Inc., the Protein Array Pioneer Company, strives to research and develop new products to meet demands of the biomedical community. RayBiotech'

s patent-pending technology allows detection of up to 1,000 cytokines, chemokines and other proteins in a single experiment. Our format is simple, sensitive, reliable, reproducible and cost-effective. Our product offerings include: 1. Protein (antigen) Arrays 2. RayBio ? Cytokine Antibody Arrays o C Series (Membrane, chemiluminescence detection) o G-Series (Glass chip, fluorescence detection) 3. Pathway- and disease-focused antibody arrays o Angiogenesis Antibody Arrays o Apoptosis Antibody Arrays o Atherosclerosis Antibody Arrays o Chemokine Antibody Arrays o Growth Factor Antibody Arrays o Inflammation Antibody Arrays o MMP Antibody Arrays o Obesity Antibody Arrays 4. Quantibody? Multiplex ELISA Arrays 5. RayBio ? L-Series Biotin Label-based Antibody Arrays 6. RayBio ? E-Series Competition-based Antibody Arrays 7. RayBio ? Phosphorylation Antibody Arrays o Receptor Tyrosine Kinases o EGFR and ErbB family (site-specific phosphorylation) 8. Over 1,000 different ELISA kits 9. EIA (Competitive ELISA) kits 10. Cell-based Phosphorylation Assay 11. Over 20,000 different Recombinant proteins 12. Peptide 13. Recombinant antibodies

1 RayBio? Human Acute Kidney Injury Antibody Array G-Series

1 Protocol for RayBio? Human Acute Kidney Injury Antibody Array G-Series

1 TABLE OF CONTENTS I. Introduction・1 II. Product Information・4 A. Storage Recommendations・4 B. Materials Provided・5 C. Additional Materials Required・5 D. How It Works

6 E. RayBio? G-Series Glass Chip Layout

6 III. Helpful Tips and General Considerations・7 A. Preparation and Storage of Samples・7 B. Handling Glass Chips・8 C. Incubations and Washes・8 D. Data Extraction Tips・9 IV. Protocol・9 A. Preparation and Storage of Reagents・9 B. Blocking and Incubations・10 C. Fluorescence Detection・14 V. Interpretation of Results・15 A. Explanation of Control Spots・15 B. Typical Results using G-Series Arrays・16 C. Background Subtraction・17 D. Normalization of Array Data・17 E. Threshold of Significance・18 VI. Antibody Array Map・19 VII. Troubleshooting Guide・20 VIII. Selected References・22 RayBio? Cytokine Antibody Arrays are patent-pending technology. RayBio? is the trademark of RayBiotech, Inc.

2 RayBio? Human Acute Kidney Injury Antibody Array G-Series

1 I. Introduction New techniques such as cDNA microarrays have enabled us to analyze global gene expression1-3 . However, almost all cell functions are executed by proteins, which cannot be studied simply through DNA and RNA techniques. Experimental analysis clearly shows disparity can exist between the relative expression levels of mRNA and their corresponding proteins4 . Therefore, analysis of the proteomic profile is critical. RayBiotech, The Protein Array Pioneer Company, introduced the first protein arrays to the market in