PDF《Pakistan Veterinary Journal》

53 Pakistan Veterinary Journal ISSN: 0253-8318 (PRINT), 2074-7764 (ONLINE) Accessible at: www.

pvj.com.pk Assessment of Buffalo Bull Semen Quality Based on Sperm Motility Parameters, Motion Characteristics and In Vitro Fertilization Rate A. Sohail, S. M. H. Andrabi1 , M. Anwar1 , L. Ali and A. Mehmood1* Department of Animal Genomics and Biotechnology, PARC Institute of Advance Studies in Agriculture, Islamabad;

1 Animal Sciences institute, National Agricultural Research Centre, Islamabad 4500, Pakistan *Corresponding Author: mehmood_apk@yahoo.com A R T I C L E H I S T O R Y A B S T R A C T Received: Revised: Accepted: November 25,

2011 May 24,

2012 June 20,

2012 Key words: Buffalo bulls CASA Cleavage rate Prognostic value The objective of the present study was to determine the prognostic value of sperm motility parameters and motion characteristics to predict in vitro fertilization (IVF) rate in the buffalo. Sperm motility parameters (%;

motile, progressive, rapid and medium) and motion characteristics (path velocity = VAP, ?m/sec;

progressive velocity = VSL, ?m/sec;

curvilinear velocity = VCL, ?m/sec;

amplitude of lateral head = ALH, ?m and beat cross frequency = BCF, Hz) of

5 buffalo bulls (Tag # 2, 4, 6,

11 and 12) were determined by Cell Motion Analyzer (CASA) at post dilution (PD), pre freezing (PF) and post thaw (PT) stages. IVF ability of individual bull was determined by cleavage rate (CR, %). Multiple regression analysis was used to determine the prognostic values of sperm motility parameters and motion characteristics for CR as a dependent variable. At PT, Bull

2 showed the highest (P